Rational Design of a Two-Photon Fluorogenic Probe for Visualizing Monoamine Oxidase A Activity in Human Glioma Tissues.
Haixiao FangHang ZhangLin LiYun NiRiri ShiZheng LiXuekang YangBo MaChengwu ZhangQiong WuChangmin YuNaidi YangShao Q YaoWei HuangPublished in: Angewandte Chemie (International ed. in English) (2020)
Monoamine oxidases have two functionally distinct but structurally similar isoforms (MAO-A and MAO-B). The ability to differentiate them by using fluorescence detection/imaging technology is of significant biological relevance, but highly challenging with available chemical tools. Herein, we report the first MAO-A-specific two-photon fluorogenic probe (F1), capable of selective imaging of endogenous MAO-A enzymatic activities from a variety of biological samples, including MAO-A-expressing neuronal SY-SY5Y cells, the brain of tumor-bearing mice and human Glioma tissues by using two-photon fluorescence microscopy (TPFM) with minimal cytotoxicity.
Keyphrases
- living cells
- single molecule
- high resolution
- endothelial cells
- fluorescent probe
- induced pluripotent stem cells
- gene expression
- induced apoptosis
- pluripotent stem cells
- quantum dots
- hydrogen peroxide
- high throughput
- energy transfer
- metabolic syndrome
- oxidative stress
- nitric oxide
- resting state
- skeletal muscle
- endoplasmic reticulum stress
- high fat diet induced
- cerebral ischemia
- white matter
- cell proliferation
- single cell
- functional connectivity
- sensitive detection