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A polyhydroxyalkanoates bioprocess improvement case study based on four fed-batch feeding strategies.

Maciej W GuzikGearóid F DuaneShane T KennyEoin CaseyPaweł MielcarekMagdalena WojnarowskaKevin E O'Connor
Published in: Microbial biotechnology (2021)
The modelling and optimization of a process for the production of the medium chain length polyhydroxyalkanoate (mcl-PHA) by the bacterium Pseudomonas putida KT2440 when fed a synthetic fatty acid mixture (SFAM) was investigated. Four novel feeding strategies were developed and tested using a constructed model and the optimum one implemented in further experiments. This strategy yielded a cell dry weight of 70.6 g l-1 in 25 h containing 38% PHA using SFAM at 5 l scale. A phosphate starvation strategy was implemented to improve PHA content, and this yielded 94.1 g l-1 in 25 h containing 56% PHA using SFAM at 5 l scale. The process was successfully operated at 20 l resulting in a cell dry weight of 91.2 g l-1 containing 65% PHA at the end of a 25-h incubation.
Keyphrases
  • single cell
  • body mass index
  • fatty acid
  • cell therapy
  • weight loss
  • physical activity
  • weight gain
  • body weight
  • stem cells
  • cystic fibrosis
  • staphylococcus aureus
  • escherichia coli
  • pseudomonas aeruginosa
  • biofilm formation