CircCYP24A1 hampered malignant phenotype of renal cancer carcinoma through modulating CMTM-4 expression via sponging miR-421.
Xiaorong WuJiale ZhouLing ZhaoZhaolin YangChen YangYonghui ChenWei XuePublished in: Cell death & disease (2022)
Renal cell carcinoma (RCC) is a lethal urinary malignancy. Circular RNAs (circRNAs) contribute to the malignant phenotype and progression of several types of human cancers, including RCC. In this study, we identified relatively low hsa_circ_0060927 (circCYP24A1) expression in RCC tissue through high-throughput sequencing and RT-qPCR. Fluorescence in situ hybridization (FISH) was used to validate the expression and subcellular localization of circCYP24A1 in RCC tissues. CCK-8, Transwell, EdU, and wound-healing assays indicated that circCYP24A1 overexpression inhibited the proliferation, invasion, and migration of RCC cells. Dual-luciferase reporter, RNA immunoprecipitation (RIP), FISH, and RNA-pulldown assays verified that circCYP24A1 inhibited RCC progression by sponging miR-421, thus inducing CMTM-4 expression. Xenograft assays and metastasis models further indicated that circCYP24A1 significantly inhibited the metastasis and proliferation of RCC cells in vivo. Taken together, circCYP24A1 is a prognosis-related circRNA in RCC that functions through the circCYP24A1/miR-421/CMTM-4 axis to modulate RCC progression.
Keyphrases
- renal cell carcinoma
- poor prognosis
- long non coding rna
- cell proliferation
- induced apoptosis
- signaling pathway
- high throughput
- long noncoding rna
- binding protein
- gene expression
- high throughput sequencing
- endothelial cells
- cell cycle arrest
- transcription factor
- oxidative stress
- squamous cell carcinoma
- cell migration
- nucleic acid
- squamous cell
- childhood cancer
- energy transfer