Phenolic Composition and Antioxidant and Antiproliferative Activities of the Extracts of Twelve Common Bean (Phaseolus vulgaris L.) Endemic Ecotypes of Southern Italy before and after Cooking.
Maria Neve OmbraAntonio d'AciernoFilomena NazzaroRiccardo RiccardiPatrizia SpignoMassimo ZaccardelliCatello PaneMena MaioneFlorinda FratianniPublished in: Oxidative medicine and cellular longevity (2016)
Beans are important dietary components with versatile health benefits. We analysed the extracts of twelve ecotypes of Phaseolus vulgaris in order to determine their phenolic profiles, antioxidant activity, and the in vitro antiproliferative activity. Ultra-performance liquid chromatography with diode array detector (UPLC-DAD) admitted us to detect and quantify some known polyphenols, such as gallic acid, chlorogenic acid, epicatechin, myricetin, formononetin, caffeic acid, and kaempferol. The antioxidant activity (AA) ranged from 1.568 ± 0.041 to 66.572 ± 3.197 mg necessary to inhibit the activity of the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical by 50% (EC50). The extracts, except those obtained from the nonpigmented samples, were capable of inhibiting the proliferation of the human epithelial colorectal adenocarcinoma (Caco-2) cells, human breast cancer cells MCF-7, and A549 NSCLC cell line. Cultivars differed in composition and concentration of polyphenols including anthocyanins; cooking affected the antioxidant activity only marginally. Qualitative and quantitative differences in phenolic composition between the groups of beans influenced the biological activities; on the other hand, we did not find significant differences on the biological activities within the same variety, before and after cooking.
Keyphrases
- breast cancer cells
- endothelial cells
- high resolution
- simultaneous determination
- liquid chromatography
- signaling pathway
- induced pluripotent stem cells
- healthcare
- induced apoptosis
- small cell lung cancer
- oxidative stress
- pluripotent stem cells
- mass spectrometry
- squamous cell carcinoma
- public health
- systematic review
- ms ms
- computed tomography
- radiation therapy
- tandem mass spectrometry
- risk assessment
- cell proliferation
- single cell