A MoS 2 -MWCNT based fluorometric nanosensor for exosome detection and quantification.
Mahnoush TayebiMohammad Tavakkoli YarakiHui Ying YangYe AiPublished in: Nanoscale advances (2019)
Circulating exosomes in body fluids are involved in many diseases and have important roles in pathophysiological processes. Specifically, they have emerged as a promising new class of biomarkers in cancer diagnosis and prognosis because of their high concentration and availability in a variety of biological fluids. The ability to quantitatively detect and characterize these nano-sized vesicles is crucial to make use of exosomes as a reliable biomarker for clinical applications. However, current methods are mostly technically challenging and time-consuming which prevents them from being adopted in clinical practice. In this work, we have developed a rapid sensitive platform for exosome detection and quantification by employing MoS 2 -multiwall carbon nanotubes as a fluorescence quenching material. This exosome biosensor shows a sensitive and selective biomarker detection. Using this MoS 2 -MWCNT based fluorometric nanosensor to analyze exosomes derived from MCF-7 breast cancer cells, we found that CD63 expression could be measured based on the retrieved fluorescence of the fluorophore with a good linear response range of 0-15% v/v. In addition, this nanosensing technique is able to quantify exosomes with different surface biomarker expressions and has revealed that exosomes secreted from MCF-7 breast cancer cells have a higher CD24 expression compared to CD63 and CD81.
Keyphrases
- breast cancer cells
- carbon nanotubes
- mesenchymal stem cells
- loop mediated isothermal amplification
- stem cells
- quantum dots
- label free
- poor prognosis
- clinical practice
- room temperature
- nk cells
- real time pcr
- sensitive detection
- energy transfer
- gold nanoparticles
- squamous cell carcinoma
- single cell
- high throughput
- highly efficient
- binding protein
- long non coding rna
- transition metal
- ionic liquid
- mouse model
- lymph node metastasis