Anti-Inflammatory and Analgesic Effects of Schisandra chinensis Leaf Extracts and Monosodium Iodoacetate-Induced Osteoarthritis in Rats and Acetic Acid-Induced Writhing in Mice.
Yun Mi LeeEunjung SonSeung-Hyung KimDong-Seon KimPublished in: Nutrients (2022)
In this study, we aimed to determine the anti-inflammatory and antinociceptive activities of Schisandra chinensis leaf extracts (SCLE) in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages, an acetic acid-induced mouse model of writhing, and a monosodium iodoacetate (MIA)-induced rat model of osteoarthritis (OA). In LPS-stimulated RAW264.7 cells, a 100 µg/mL dose of SCLE significantly reduced the production of nitric oxide (NO), interleukin-1β (IL-1β), tumour necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), and prostaglandin E2 (PGE2). Acetic acid-induced writhing responses in mice that quantitatively determine pain were significantly inhibited by SCLE treatment. In addition, SCLE significantly decreased the MIA-induced elevation in OA symptoms, the expression levels of pro-inflammatory mediators/cytokines and matrix metalloproteinases, and cartilage damage in the serum and joint tissues. Our data demonstrated that SCLE exerts anti-osteoarthritic effects by regulating inflammation and pain and can be a useful therapeutic candidate against OA.
Keyphrases
- anti inflammatory
- knee osteoarthritis
- high glucose
- nitric oxide
- diabetic rats
- mouse model
- rheumatoid arthritis
- oxidative stress
- chronic pain
- inflammatory response
- neuropathic pain
- induced apoptosis
- drug induced
- pain management
- poor prognosis
- gene expression
- spinal cord injury
- cell death
- immune response
- machine learning
- adipose tissue
- big data
- electronic health record
- depressive symptoms
- skeletal muscle
- sleep quality
- data analysis