A Cost-effective, High-throughput, Highly Accurate Genotyping Method for Outbred Populations.

Denghui Chen Apurva S Chitre Khai-Minh H Nguyen Katarina Cohen Beverly Peng Kendra S Ziegler Faith OkamotoBonnie LinBenjamin B Johnson Thiago Missfeldt Sanches Riyan Cheng Oksana Polesskaya Abraham A Palmer

Published in: bioRxiv : the preprint server for biology (2024)

Heterogeneous stock rats were derived by interbreeding eight inbred founders in 1984, and have been maintained as an outbred population for more than 100 generations. Heterogeneous stock rats offer a high degree of genetic and phenotypic diversity, and have been extensively used for genetic studies. Here, we describe a cost-effective and high-throughput genotyping method for heterogeneous stock rats. We applied the method to 15,552 heterogeneous stock rats. The resulting genotypes were highly accurate. These techniques may be useful for genetic studies in other non-human subjects.

Keyphrases

gene expression
high throughput
genome wide
dna methylation
copy number
endothelial cells
single cell
high resolution
multidrug resistant
genetic diversity
mass spectrometry
induced pluripotent stem cells
pluripotent stem cells