2'-Fucosyllactose Inhibits Human Norovirus Replication in Human Intestinal Enteroids.
Ketki PatilB Vijayalakshmi AyyarFrederick H NeillLars BodeMary K EstesRobert L AtmarSasirekha RamaniPublished in: bioRxiv : the preprint server for biology (2024)
Human noroviruses infect the gastrointestinal tract and are a leading cause of acute gastroenteritis worldwide. Common symptoms of norovirus include diarrhea, vomiting and stomach cramps. Virus shedding and symptoms are prolonged and debilitating in immunocompromised patients. Currently, there are no approved vaccines or targeted antivirals for treating human norovirus infection. Human intestinal enteroids derived from intestinal stem cells allow the successful replication of norovirus in the laboratory and can be used as a physiologically relevant model system to evaluate antivirals. We discovered that 2'fucosyllactose (2'FL), an oligosaccharide naturally occurring in human milk, inhibits norovirus replication in HIEs from multiple donors and thus has the potential to be developed as a therapeutic for human norovirus. These findings have high translational potential since 2'FL from several manufacturers have GRAS (generally recognized as safe) status and can be synthesized on a large scale for immediate application.
Keyphrases
- kidney transplantation
- endothelial cells
- stem cells
- human milk
- induced pluripotent stem cells
- pluripotent stem cells
- intensive care unit
- end stage renal disease
- mesenchymal stem cells
- physical activity
- depressive symptoms
- transcription factor
- newly diagnosed
- preterm infants
- peritoneal dialysis
- sleep quality
- extracorporeal membrane oxygenation
- drug induced
- preterm birth
- clostridium difficile