Novel CRISPR/Cas9-mediated knockout of LIG4 increases efficiency of site-specific integration in Chinese hamster ovary cell line.

Chuanjie WangZhaolin SunMing WangZhiyang JiangMengmeng ZhangHongxu CaoLonglong LuoChunxia QiaoHe XiaoGuojiang ChenXinying LiJinqing LiuZeliang WeiBeifen ShenJing WangJiannan Feng

Published in: Biotechnology letters (2022)

Deficiency of LIG4 represents a feasible strategy to improve SSI efficiency and suggests it can be applied to develop and engineer CHO cell lines in the future.

Keyphrases

crispr cas
genome editing
surgical site infection
current status
replacement therapy
wild type