Number and function of circulatory helper innate lymphoid cells are unaffected by immunosuppressive drugs used in solid organ recipients - a single centre cohort study.
Elena Gómez-MassaPaloma TalayeroAlberto Utrero-RicoRocío Laguna-GoyaAmado AndrésEsther ManceboAlejandra LeivasNatalia Polanco-FernándezIago JustoCarlos Jimenez-RomeroDaniel PleguezueloEstela Paz-ArtalPublished in: Transplant international : official journal of the European Society for Organ Transplantation (2020)
In transplanted intestines, depletion of T cells together with long-term persistence of ILC is observed, suggesting ILC insensitivity to immunosuppressive drugs. To further analyze helper ILC (hILC) apparent resistance to therapy, cytotoxic ILC (NK cells), hILC subsets (ILC1, ILC2, and ILC precursors (ILCP)), and their signature cytokines (IFNγ, IL4 + IL13, and IL22) were analyzed in peripheral blood of kidney and liver transplant recipients. Early after transplantation (posTx), transplanted patients showed significantly lower Lin + and NK cells, whereas total hILC, ILC1, ILC2, and ILCP numbers were similar in patients and controls. Between paired pre- and posTx samples, Lin + cell and NK cell counts significantly decreased, whereas all three hILC counts and their cytokine production remained similar. ILC1, ILC2, and ILCP numbers were also similar in patients under thymoglobulin or basiliximab (BAS), patients without induction (only maintenance therapy) and controls. hILC showed lower TMG binding comparing to Lin + cells, reduced expression of CD25 (BAS target), and diminished calcineurin activity with undetectable calcineurin and FKBP12 (tacrolimus target). hILC counts were not related to delayed graft function or biopsy-proven acute rejection. Thus, hILC remain stable early after transplantation and seem unaffected by immunosuppressors, which may be related to reduced targets expression and low calcineurin activity.
Keyphrases
- nk cells
- end stage renal disease
- peripheral blood
- ejection fraction
- newly diagnosed
- chronic kidney disease
- prognostic factors
- peritoneal dialysis
- poor prognosis
- immune response
- dendritic cells
- induced apoptosis
- magnetic resonance imaging
- regulatory t cells
- signaling pathway
- extracorporeal membrane oxygenation
- hepatitis b virus
- bone marrow
- liver failure
- acute respiratory distress syndrome
- fine needle aspiration
- anti inflammatory