Fortifying angiogenic efficacy of conditioned media using phototoxic-free blue light for wound healing.

We used a blue organic light-emitting diode (bOLED) to increase the paracrine factors secreted from human adipose-derived stem cells (hADSCs) for producing conditioned medium (CM). Our results showed that while the bOLED irradiation promotes a mild-dose reactive oxygen generation that enhances the angiogenic paracrine secretion of hADSCs, it does not induce phototoxicity. The bOLED enhances paracrine factors via a cell-signaling mechanism involving hypoxia-inducible factor 1 alpha. This study demonstrated that the CM resulting from bOLED treatment shows improved therapeutic effects on mouse wound-healing models. This method contributes to overcoming the barriers to stem-cell therapies, including the toxicity and low yields from other methods such as nanoparticles, synthetic polymers, and even cell-derived vesicles.