Altered Lipid Composition of Secretory Cells Following Exposure to Zinc Can Be Correlated to Changes in Exocytosis.
Lin RenMasoumeh Dowlatshahi PourPer MalmbergAndrew G EwingPublished in: Chemistry (Weinheim an der Bergstrasse, Germany) (2019)
A micromolar concentration of zinc has been shown to significantly change the dynamics of exocytosis as well as the vesicle contents in a model cell line, providing direct evidence that zinc regulates neurotransmitter release. To provide insight into how zinc modulates these exocytotic processes, neurotransmitter release and vesicle content were compared with single cell amperometry and intracellular impact vesicle cytometry with a range of zinc concentrations. Additionally, time-of-flight secondary ion mass spectrometry (ToF-SIMS) images of lipid distributions in the cell membrane after zinc treatment correlate to changes in exocytosis. By combining electrochemical techniques and mass spectrometry imaging, we proposed a mechanism by which zinc changes the fusion pore and the rate of neurotransmitter release by changing lipid distributions and results in the modulation of synaptic strength and plasticity.
Keyphrases
- mass spectrometry
- oxide nanoparticles
- single cell
- high resolution
- liquid chromatography
- gold nanoparticles
- fatty acid
- induced apoptosis
- signaling pathway
- high performance liquid chromatography
- photodynamic therapy
- ionic liquid
- combination therapy
- gas chromatography
- cell cycle arrest
- pi k akt
- molecularly imprinted
- fluorescence imaging
- simultaneous determination