The P2X7 Receptor is a Master Regulator of Microparticle and Mitochondria Exchange in Mouse Microglia.
Simonetta FalzoniValentina Vultaggio-PomaPaola ChiozziMario TarantiniElena AdinolfiPaola BoldriniAnna Lisa GiulianiGiampaolo MorcianoYong TangDariusz C GóreckiFrancesco Di VirgilioPublished in: Function (Oxford, England) (2024)
Microparticles (MPs) are secreted by all cells, where they play a key role in intercellular communication, differentiation, inflammation, and cell energy transfer. P2X7 receptor (P2X7R) activation by extracellular ATP (eATP) causes a large MP release and affects their contents in a cell-specific fashion. We investigated MP release and functional impact in microglial cells from P2X7R-WT or P2X7R-KO mice, as well as mouse microglial cell lines characterized for high (N13-P2X7RHigh) or low (N13-P2X7RLow) P2X7R expression. P2X7R stimulation promoted release of a mixed MP population enriched with naked mitochondria. Released mitochondria were taken up and incorporated into the mitochondrial network of the recipient cells in a P2X7R-dependent fashion. NLRP3 and the P2X7R itself were also delivered to the recipient cells. Microparticle transfer increased the energy level of the recipient cells and conferred a pro-inflammatory phenotype. These data show that the P2X7R is a master regulator of intercellular organelle and MP trafficking in immune cells.
Keyphrases
- induced apoptosis
- cell cycle arrest
- cell death
- oxidative stress
- inflammatory response
- poor prognosis
- endoplasmic reticulum stress
- lipopolysaccharide induced
- mass spectrometry
- reactive oxygen species
- machine learning
- transcription factor
- cell proliferation
- energy transfer
- spinal cord
- high resolution
- bone marrow
- pi k akt
- long non coding rna
- deep learning
- endoplasmic reticulum