Sesquiterpenoids from the Mangrove-Derived Aspergillus ustus 094102.

Four new drimane sesquiterpenoids ( 1 - 4 ) and three known ones ( 5 - 7 ) were isolated from the fermentation broth of the mangrove-derived Aspergillus ustus 094102. Compound 5 was further resolved as four purified compounds 5a - 5d . By means of extensive spectroscopic and ECD analysis as well as the chemical transformation, their structures were identified as (2 R ,3 R ,5 S ,9 R ,10 S )-2,3,9,11-tetrahydroxydrim-7-en-6-one (ustusol F, 1 ), (2 R ,3 R ,5 R ,9 S ,10 R )-2,3,11-trihydroxydrim-7-en-6-one (9-deoxyustusol F, 2 ), (3 S ,5 R ,9 R ,10 R )-3,11,12-trihydroxydrim-7-en-6-one (ustusol G, 3 ), (5 S ,6 R ,9 S ,10 S , 11 R ,2' E ,4' E )-(11-dideoxy-11-hydroxystrobilactone A-6-yl)-5-carboxypenta-2,4-dienoate (ustusolate H, 4 ), ((5 S ,6 R ,9 S ,10 S )-strobilactone A-6-yl) (2 E ,4 E )-6,7-dihydroxyocta-2,4-dienoate (ustusolate I, 5 ), (2' E ,4' E ;6',7'- erythro )-ustusolate I ( 5a ) and (2' E ,4' E ; ent -6',7'- erythro )-ustusolate I ( 5b ), (2' E ,4' E ,6' R ,7' R )-ustusolate I ( 5c ) and (2' E ,4' E ,6' S ,7' S )-ustusolate I ( 5d ), (5 S ,6 R ,9 S ,10 S ,2' E ,4' E )-(strobilactone A-6-yl)-5-carboxypenta-2,4-dienoate (ustusolate J, 6 ), and (2 S ,5 S ,9 R ,10 S )-2,9,11-trihydroxydrim-7-en-6-one (ustusol B, 7 ), respectively. Compound 5 showed antiproliferation against the human tumor cells CAL-62 and MG-63 with the IC 50 values of 16.3 and 10.1 µM, respectively.