Hsa_circ_0092856 Promoted the Proliferation, Migration, and Invasion of NSCLC Cells by Up-Regulating the Expression of eIF3a.
Fuqiang YuanMasha HuangHanxue HuangXiao-Yuan MaoPan XieXi LiYang GaoFeiyue ZengZhaoqian LiuPublished in: Biomedicines (2024)
Circular RNA (circRNA) plays a very important regulatory role in a variety of human malignancies such as non-small-cell lung cancer (NSCLC). In the current study, we explored the role of hsa_circ_0092856 in the progression of NSCLC. We screened CircRNA from the eIF3a gene in the Circbase database. The biological functions of hsa_circ_0092856 in NSCLC were analyzed via qRT-PCR, a CCK-8 assay, a plate cloning experiment, scratch testing, a transwell chamber experiment, an RNA nuclear mass separation experiment, an RIP experiment, and a Western blot test. The results showed that hsa_circ_0092856 was highly expressed in NSCLC cells, and the knockdown of hsa_circ_0092856 could inhibit the proliferation, migration, and invasion of NSCLC cells. The overexpression of hsa_circ_0092856 has the opposite effect. The expression of eIF3a also changed with the change in hsa_circ_0092856. These results suggest that hsa_circ_0092856 may play a key role in the progression of NSCLC by regulating the expression of eIF3a.
Keyphrases
- small cell lung cancer
- advanced non small cell lung cancer
- induced apoptosis
- poor prognosis
- cell cycle arrest
- brain metastases
- signaling pathway
- endothelial cells
- endoplasmic reticulum stress
- epidermal growth factor receptor
- cell death
- binding protein
- cell proliferation
- emergency department
- oxidative stress
- south africa
- transcription factor
- gene expression
- dna methylation
- genome wide
- copy number
- induced pluripotent stem cells