Practical optimization of liquid chromatography/mass spectrometry conditions and pretreatment methods toward the sensitive quantification of auxin in plants.
Kohtaro SugaharaKazunori KitaoTohru YamagakiTomotsugu KoyamaPublished in: Rapid communications in mass spectrometry : RCM (2020)
Our practical optimization of LC/MS conditions and pretreatment methods provides detailed experimental processes toward the sensitive quantification of auxin from 10 mg of plant tissue. These processes have not always been clearly documented; therefore, our protocol could broadly contribute to technical advances in plant growth and development research.