The Effects of the Follicle-Stimulating Hormone on Human Follicular Fluid-Derived Stromal Cells.
Giedrė SkliutėBrigita Vaigauskaitė-MažeikienėAlgirdas KaupinisMindaugas ValiusEdita KazėnaitėRūta NavakauskienėPublished in: International journal of molecular sciences (2023)
The prevalence of infertility is getting higher over the years. The increasing age of first-time parents, although economically more desirable, can cause various biological problems from low natural conception rate to poor pregnancy outcomes. The growing demand for assisted reproductive technology procedures worldwide draws medical specialists' and scientists' attention to various elements which could lead to successful conception, such as follicular fluid (FF) and hormones. In this study, we analyzed the effects of exposure to follicle-stimulating hormone (FSH) on FF-derived stromal cells isolated from females admitted for treatment due to infertility, participating in assisted reproductive technologies procedures. We demonstrated that FF stromal cells are positive for mesenchymal stromal cell surface markers (CD90 + , CD44 + , CD166 + ) and showed that FSH has no impact on FF stromal cell morphology yet lowers proliferation rate. Using a real-time polymerase chain reaction method, we indicated that the expression of PTGS2 is significantly downregulated in FF sediment cells of patients who did not conceive; furthermore, we showed that FSH can affect the expression of ovarian follicle development and FSH response-related genes differentially depending on the length of exposure and that levels of ovulatory cascade genes differ in conceived and not-conceived patients' FF stromal cells. Using mass spectrometry analysis, we identified 97 proteins secreted by FF stromal cells. The identified proteins are related to stress response, positive regulation of apoptotic cell clearance and embryo implantation.
Keyphrases
- pregnancy outcomes
- bone marrow
- mass spectrometry
- poor prognosis
- cell surface
- single cell
- end stage renal disease
- healthcare
- ejection fraction
- cell therapy
- induced apoptosis
- endothelial cells
- cell death
- pregnant women
- newly diagnosed
- signaling pathway
- stem cells
- chronic kidney disease
- mental health
- gene expression
- risk factors
- metabolic syndrome
- polycystic ovary syndrome
- high resolution
- liquid chromatography
- oxidative stress
- dna methylation
- prognostic factors
- genome wide
- adipose tissue
- type diabetes
- heavy metals
- endoplasmic reticulum stress
- combination therapy
- smoking cessation
- nk cells
- capillary electrophoresis
- organic matter