Plasmid encoding miRNA-200c delivered by CaCO 3 -based nanoparticles enhances rat alveolar bone formation.

Aim: miRNAs have been shown to improve the restoration of craniofacial bone defects. This work aimed to enhance transfection efficiency and miR-200c -induced bone formation in alveolar bone defects via plasmid DNA encoding miR-200c delivery from CaCO 3 nanoparticles. Materials & methods: The CaCO 3 / miR-200c delivery system was evaluated in vitro (microscopy, transfection efficiency, biocompatibility) and miR-200c -induced in vivo alveolar bone formation was assessed via micro-computed tomography and histology. Results: CaCO 3 nanoparticles significantly enhanced the transfection of plasmid DNA encoding miR-200c without inflammatory effects and sustained miR-200c expression. CaCO 3 / miR-200c treatment in vivo significantly increased bone formation in rat alveolar bone defects. Conclusion: CaCO 3 nanoparticles enhance miR-200c delivery to accelerate alveolar bone formation, thereby demonstrating the application of CaCO 3 / miR-200c to craniofacial bone defects.