Green-synthesized copper oxide nanoparticles induce apoptosis and up-regulate HOTAIR and HOTTIP in pancreatic cancer cells.
Zahra HosseiniAmirhossein AhmadiAhmad ShadiSeyed Javad HosseiniHossein NikmaneshPublished in: Nanomedicine (London, England) (2024)
Aim: Cu 2 O nanoparticles were synthesized using an extract from S. latifolium algae (SLCu 2 O NPs). Their effect on PANC-1 cells and the expression of two drug resistance-related lncRNAs were evaluated in comparison with Arsenic trioxide. Materials & methods: SLCu 2 O NPs were characterized using XRD, SEM, and TEM microscopies. The effects of SLCu 2 O NPs on cell cytotoxicity, cell cycle, and apoptosis, and expression of two drug resistance-related lncRNAs were examined using MTT assay, flow cytometry, and real-time PCR, respectively. Results: SLCu 2 O NPs demonstrated anti-cancer properties against PANC-1 cells comparable to Arsenic trioxide, and the expression of lncRNAs increased upon treatment with them. Conclusion: SLCu 2 O NPs demonstrate anti-cancer properties against PANC-1 cells; however, using gene silencing strategies along with SLCu 2 O NPs is suggested.
Keyphrases
- oxide nanoparticles
- cell cycle arrest
- induced apoptosis
- cell cycle
- cell death
- oxidative stress
- poor prognosis
- endoplasmic reticulum stress
- flow cytometry
- pi k akt
- cell proliferation
- signaling pathway
- drinking water
- stem cells
- real time pcr
- high throughput
- single cell
- genome wide identification
- walled carbon nanotubes