Sensitive and rapid detection of Escherichia coli O157:H7 from beef sample based on recombinase aided amplification assisted CRISPR/Cas12a system.

Taisong Fang Jinling Shen Junxin Xue Yuan Jiang Dehua Guo Jielin Yang Xiangxiang Kong Xuebin Xu Xiang Wang

Published in: Journal of AOAC International (2022)

The RAA-CRISPR/Cas12a system proposed in this study provided an alternative molecular tool for quick, specific, sensitive and accurate detection of E. coli O157: H7 in foods.

Keyphrases

crispr cas
escherichia coli
genome editing
high resolution
nucleic acid
klebsiella pneumoniae