Optical Pacing of Human-Induced Pluripotent Stem Cell-Derived Cardiomyocytes Mediated by a Conjugated Polymer Interface.
Francesco LodolaVito VurroSilvia CrastoElisa Di PasqualeGuglielmo LanzaniPublished in: Advanced healthcare materials (2019)
The use of light for triggering skeletal and cardiac muscles allows lower invasiveness higher selectivity and unprecedented possibility to target individual cells or even subcellular compartments in a temporally and spatially precise manner. Because cells are in general transparent, this requires the development of suitable interfaces that bestow light sensitivity to living matter. In the present work, successfully demonstrated is the use of conjugated polymer films as transducer to optically enhance the contraction rate of a human and patient-specific cardiac in vitro cell model. By different experimental approaches, the coupling mechanism to the photothermal effect is assigned. This work extends the range of application of the polymer-mediated cell photostimulation phenomenon to cardiac muscle cells, opening up possible applications in cardiac therapy and for implementation of in vitro studies.
Keyphrases
- induced apoptosis
- cell cycle arrest
- endothelial cells
- left ventricular
- photodynamic therapy
- single cell
- cell therapy
- stem cells
- primary care
- healthcare
- high glucose
- endoplasmic reticulum stress
- signaling pathway
- drug delivery
- induced pluripotent stem cells
- room temperature
- pluripotent stem cells
- cardiac resynchronization therapy
- pi k akt
- atrial fibrillation
- cell proliferation
- structural basis
- stress induced