Putative Integrative Mobile Elements That Exploit the Xer Recombination Machinery Carrying blaIMI-Type Carbapenemase Genes in Enterobacter cloacae Complex Isolates in Singapore.

Tse H KohNurdyana Binte Abdul RahmanJeanette W P TeoMy-Van LaBalamurugan PeriaswamySwaine L Chen

Published in: Antimicrobial agents and chemotherapy (2017)

Whole-genome sequencing was performed on 16 isolates of the carbapenemase-producing Enterobacter cloacae complex to determine the flanking regions of blaIMI-type genes. Phylogenetic analysis of multilocus sequence typing (MLST) targets separated the isolates into 4 clusters. The blaIMI-type genes were all found on Xer-dependent integrative mobile elements (IMEX). The IMEX elements of 5 isolates were similar to those described in Canada, while the remainder were novel. Five isolates had IMEX elements lacking a resolvase and recombinase.

Keyphrases

genetic diversity
genome wide
klebsiella pneumoniae
genome wide identification
bioinformatics analysis
dna damage
gram negative
gene expression
oxidative stress
dna repair
cystic fibrosis
network analysis
pseudomonas aeruginosa