Cysteine Disulfides (Cys-ss-X) as Sensitive Plasma Biomarkers of Oxidative Stress.
Xiaoyun FuShelby A CateMelissa DominguezWarren OsbornTahsin ÖzpolatBarbara A KonkleJunmei ChenJosé A LópezPublished in: Scientific reports (2019)
We developed a high-throughput mass spectrometry-based method to simultaneously quantify numerous small-molecule thiols and disulfides in blood plasma. Application of this assay to analyze plasma from patients with known oxidative stress (sickle cell disease and sepsis) and from a patient with sickle cell disease treated with the antioxidant N-acetylcysteine suggests that cysteine disulfides, in particular protein-bound cysteine, serve as sensitive plasma biomarkers for the extent of oxidative stress and effectiveness of antioxidant treatment.
Keyphrases
- oxidative stress
- high throughput
- small molecule
- sickle cell disease
- mass spectrometry
- dna damage
- diabetic rats
- ischemia reperfusion injury
- induced apoptosis
- randomized controlled trial
- systematic review
- intensive care unit
- living cells
- protein protein
- anti inflammatory
- case report
- high resolution
- ms ms
- combination therapy
- amino acid
- simultaneous determination