Mechanism of delayed cell death following simultaneous CRISPR-Cas9 targeting in pancreatic cancers.

Selina Shiqing K TehEitan Halper-StrombergLaura MorsbergerAlexis BennettKirsten BowlandAlyza SkaistFidel CaiHong LiangRalph H HrubanNicholas J RobertsRobert B ScharpfYing S ZouJames R Eshleman

Published in: bioRxiv : the preprint server for biology (2023)

Using whole genome sequencing and conventional cytogenetics, we discovered that CRISPR-Cas9 cuts led to genomic instability, including chromosomal rearrangements and polyploidization, and ultimately to delayed cell death, 2-3 weeks after the induction of CRISPR-Cas9 DSBs.

Keyphrases

crispr cas
cell death
genome editing
copy number
cell cycle arrest
dna methylation
signaling pathway
drug delivery
pi k akt