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Mechanism of delayed cell death following simultaneous CRISPR-Cas9 targeting in pancreatic cancers.

Selina Shiqing K TehEitan Halper-StrombergLaura MorsbergerAlexis BennettKirsten BowlandAlyza SkaistFidel CaiHong LiangRalph H HrubanNicholas J RobertsRobert B ScharpfYing S ZouJames R Eshleman
Published in: bioRxiv : the preprint server for biology (2023)
Using whole genome sequencing and conventional cytogenetics, we discovered that CRISPR-Cas9 cuts led to genomic instability, including chromosomal rearrangements and polyploidization, and ultimately to delayed cell death, 2-3 weeks after the induction of CRISPR-Cas9 DSBs.
Keyphrases
  • crispr cas
  • cell death
  • genome editing
  • copy number
  • cell cycle arrest
  • dna methylation
  • signaling pathway
  • drug delivery
  • pi k akt