Validating the Health Benefits of Coffee Berry Pulp Extracts in Mice with High-Fat Diet-Induced Obesity and Diabetes.
Khawaja Muhammad Imran BashirJoo Wan KimHye-Rim ParkJae-Kyoung LeeBeom-Rak ChoiJae-Suk ChoiSae Kwang KuPublished in: Antioxidants (Basel, Switzerland) (2023)
The effects of coffee ( Coffea arabica L.) berry pulp extracts (CBP extracts) on the improvement of diabetes, obesity, and non-alcoholic fatty liver disease (NAFLD) were evaluated using various in vitro antioxidant activity assays and through a high-fat diet-induced mild diabetic obese mouse model. After an 84-day oral administration of CBP extracts (400-100 mg/kg), bioactivities were evaluated. The in vitro analysis showed the highest DPPH ● scavenging activity of 73.10 ± 4.27%, ABTS ● scavenging activity of 41.18 ± 1.14%, and SOD activity of 56.24 ± 2.81%, at a CBP extract concentration of 1000 µg/mL. The in vivo analysis of the CBP extracts showed favorable and dose-dependent anti-obesity, anti-diabetic, NAFLD, nephropathy, and hyperlipidemia refinement effects through hepatic glucose enzyme activity, 5'-AMP-activated protein kinase (AMPK) up-regulation, antioxidant activity, lipid metabolism-related gene expression, and pancreatic lipid digestion enzyme modulatory activities. This study shows that an appropriate oral dosage of CBP extracts could function as a potent herbal formulation for a refinement agent or medicinal food ingredient to control type 2 diabetes and related complications.
Keyphrases
- high fat diet induced
- type diabetes
- insulin resistance
- gene expression
- protein kinase
- glycemic control
- adipose tissue
- mouse model
- high fat diet
- cardiovascular disease
- metabolic syndrome
- skeletal muscle
- healthcare
- public health
- weight loss
- dna methylation
- mental health
- oxidative stress
- fatty acid
- human health
- blood glucose
- drug delivery
- bariatric surgery
- health information
- risk factors
- single cell
- high throughput
- drug induced
- weight gain