iJAZ-based approach to engineer lepidopteran pest resistance in multiple crop species.
Huijuan MoHuimin ChangGe ZhaoGuanjing HuXiumei LuoXue JiaZhenlu XuGuangming RenLi FengJonathan F WendelXiao-Ya ChenMaozhi RenFuguang LiPublished in: Nature plants (2024)
The fall armyworm (FAW) poses a significant threat to global crop production. Here we showed that overexpression of jasmonate ZIM-domain (JAZ) protein GhJAZ24 confers resistance to cotton bollworm and FAW, while also causing sterility in transgenic cotton by recruiting TOPLESS and histone deacetylase 6. We identified the NGR motif of GhJAZ24 that recognizes and binds the aminopeptidase N receptor, enabling GhJAZ24 to enter cells and disrupt histone deacetylase 3, leading to cell death. To overcome plant sterility associated with GhJAZ24 overexpression, we developed iJAZ (i, induced), an approach involving damage-induced expression and a switch from intracellular to extracellular localization of GhJAZ24. iJAZ transgenic cotton maintained fertility and showed insecticidal activity against cotton bollworm and FAW. In addition, iJAZ transgenic rice, maize and tobacco plants showed insecticidal activity against their lepidopteran pests, resulting in an iJAZ-based approach for generating alternative insecticidal proteins with distinctive mechanisms of action, thus holding immense potential for future crop engineering.
Keyphrases
- histone deacetylase
- climate change
- cell death
- high glucose
- cell cycle arrest
- diabetic rats
- cell proliferation
- induced apoptosis
- oxidative stress
- poor prognosis
- binding protein
- transcription factor
- endothelial cells
- signaling pathway
- drug induced
- risk assessment
- human health
- young adults
- endoplasmic reticulum stress
- long non coding rna
- childhood cancer