In vitro chemo-protective effect of Eisenia foetida coelomic fluid against histone deacetylase inhibitor-induced oxidative toxicity in breast cancer cells.
Asuman Deveci OzkanJaniah AlimudinYasemin KilcilerBurcu YukselOzlem AksoyZeynep BettsPublished in: International journal of environmental health research (2022)
Developing new drugs from natural products is important for therapeutic effects to minimise tissue toxicity of drugs used in cancer treatment. Eisenia foetida is a worm with a double transport system consisting of coelomic fluid (ECF) that can be used as alternative medicine. It is important to eliminate or reduce the high cytotoxicity of sodium butyrate (NaBu), a chemotherapeutic agent used in breast cancer treatment, for both neoplastic and normal cells. We aimed to evaluate the effect of ECF on the oxidative toxicity induced by NaBu in MCF-7 cells, changes in ROS production levels and expression of cell death and ROS-related genes. It was determined that the expression levels of Bax and Bcl-2 genes remained unchanged, while the amount of ROS decreased significantly in MCF-7 cells exposed to NaBu with ECF. Thus, ECF may be a potential therapeutic molecule with fewer side effects in cancer treatment in the future.
Keyphrases
- cell death
- cell cycle arrest
- induced apoptosis
- breast cancer cells
- oxidative stress
- poor prognosis
- dna damage
- endoplasmic reticulum stress
- reactive oxygen species
- squamous cell carcinoma
- gene expression
- dna methylation
- cell proliferation
- genome wide
- diabetic rats
- long non coding rna
- current status
- transcription factor