Development of a high-throughput γ-H2AX assay based on imaging flow cytometry.

Younghyun LeeQi WangIgor ShuryakDavid J BrennerHelen C Turner

Published in: Radiation oncology (London, England) (2019)

The results indicate that the IFC-based γ-H2AX protocol may provide a practical and high-throughput platform for measurements of individual global DNA DSB repair capacity which can facilitate precision medicine by predicting individual radiosensitivity and risk of developing adverse effects related to radiotherapy treatment.

Keyphrases

high throughput
flow cytometry
single cell
high resolution
early stage
randomized controlled trial
circulating tumor
radiation therapy
cell free
radiation induced
locally advanced
squamous cell carcinoma
combination therapy