Phosphorylation of ETS-1 is a critical event in DNA polymerase iota-induced invasion and metastasis of esophageal squamous cell carcinoma.
Chao HeShuhua WuAidi GaoYe SuHan MinZeng-Fu ShangJinchang WuLi YangWei-Qun DingJundong ZhouPublished in: Cancer science (2017)
An aberrantly elevated expression of DNA polymerase ι (Pol ι) is significantly associated with poor prognosis of patients with esophageal squamous cell carcinoma (ESCC), yet the mechanisms behind this phenomenon remain obscure. Based on the RNA-Seq transcriptome and real-time PCR analysis, we identified ETS-1 as a candidate gene involved in Pol ι-mediated progression of ESCC. Wound-healing and transwell assay indicated that downregulation of ETS-1 attenuates Pol ι-mediated invasiveness of ESCC. Signaling pathway analysis showed that Pol ι enhances ETS-1 phosphorylation at threonine-38 through the Erk signaling pathway in ESCC cells. Kaplan-Meier analysis, based on 93 clinical tissue samples, revealed that ETS-1 phosphorylation at threonine-38 is associated with poor prognosis of ESCC patients. The present study thus demonstrates that phosphorylation of ETS-1 is a critical event in the Pol ι-induced invasion and metastasis of ESCC.
Keyphrases
- poor prognosis
- signaling pathway
- transcription factor
- rna seq
- long non coding rna
- protein kinase
- single cell
- induced apoptosis
- pi k akt
- end stage renal disease
- genome wide
- gene expression
- epithelial mesenchymal transition
- ejection fraction
- chronic kidney disease
- circulating tumor
- wound healing
- cell free
- newly diagnosed
- real time pcr
- prognostic factors
- single molecule