Quadruplex-forming oligonucleotide targeted to the VEGF promoter inhibits growth of non-small cell lung cancer cells.
David E MuenchFrancine RezzougShelia D ThomasJingjing XiaoAshraful IslamDonald M MillerKara C SedorisPublished in: PloS one (2019)
Our data strongly suggest that the G-rich VEGFq oligonucleotide binds specifically to the C-rich strand of the genomic VEGF promoter, via strand invasion, stabilizing the quadruplex structure formed by the genomic G-rich sequence, resulting in transcriptional inhibition. Strand invading oligonucleotides represent a new approach to specifically inhibit VEGF expression that avoids many of the problems which have plagued the therapeutic use of oligonucleotides. This is a novel approach to specific inhibition of gene expression.
Keyphrases
- gene expression
- vascular endothelial growth factor
- dna methylation
- endothelial cells
- transcription factor
- induced apoptosis
- copy number
- poor prognosis
- mental health
- electronic health record
- cell cycle arrest
- genome wide
- cancer therapy
- cell migration
- binding protein
- big data
- cell proliferation
- signaling pathway
- artificial intelligence
- heat shock
- heat stress