Zona pellucida treatment before CRISPR/Cas9-mediated genome editing of porcine zygotes.
Zhao NamulaMaki HirataQuynh Anh LeQingyi LinKoki TakebayashiNaoaki YoshimuraFuminori TaniharaChommanart ThongkittidilokTakeshige OtoiPublished in: Veterinary medicine and science (2021)
Our results indicate that weakening the ZP does not affect the developmental competence, mutation rate, or mutation efficiency of electroporated zygotes, whereas ZP removal has a detrimental effect on embryonic development but may increase the mutation rate.