Altered Differential Expression of Genes and microRNAs Related to Adhesion and Apoptosis Pathways in Patients with Different Phenotypes of Endometriosis.
Luana Grupioni Lourenço AntonioJuliana MeolaAna Carolina Japur de Sá Rosa-E-SilvaAntonio Alberto NogueiraFrancisco José Candido Dos ReisOmero Benedicto Poli NetoJulio Cesar Rosa E SilvaPublished in: International journal of molecular sciences (2023)
We aim to investigate the expression of genes ( MAPK1 and CAPN2 ) and microRNAs (miR-30a-5p, miR-7-5p, miR-143-3p, and miR-93-5p) involved in adhesion and apoptosis pathways in superficial peritoneal endometriosis (SE), deep infiltrating endometriosis (DE), and ovarian endometrioma (OE), and to evaluate whether these lesions share the same pathophysiological mechanisms. We used samples of SE ( n = 10), DE ( n = 10), and OE ( n = 10), and endometrial biopsies of these respective patients affected with endometriosis under treatment at a tertiary University Hospital. Endometrial biopsies collected in the tubal ligation procedure from women without endometriosis comprised the control group ( n = 10). Quantitative real-time polymerase chain reaction was performed. The expression of MAPK1 ( p < 0.0001), miR-93-5p ( p = 0.0168), and miR-7-5p ( p = 0.0006) was significantly lower in the SE group than in the DE and OE groups. The expression of miR-30a ( p = 0.0018) and miR-93 ( p = 0.0052) was significantly upregulated in the eutopic endometrium of women with endometriosis compared to the controls. MiR-143 ( p = 0.0225) expression also showed a statistical difference between the eutopic endometrium of women with endometriosis and the control group. In summary, SE showed lower pro-survival gene expression and miRNAs involved in this pathway, indicating that this phenotype has a different pathophysiological mechanism compared to DE and OE.
Keyphrases
- poor prognosis
- gene expression
- oxidative stress
- signaling pathway
- binding protein
- genome wide
- end stage renal disease
- cell death
- prognostic factors
- pregnant women
- ultrasound guided
- polycystic ovary syndrome
- minimally invasive
- mass spectrometry
- biofilm formation
- staphylococcus aureus
- cell proliferation
- smoking cessation
- replacement therapy