Differential regulation by CD47 and thrombospondin-1 of extramedullary erythropoiesis in mouse spleen.
Rajdeep BanerjeeThomas J MeyerMargaret C CamSukhbir KaurDavid D RobertsPublished in: eLife (2024)
Extramedullary erythropoiesis is not expected in healthy adult mice, but erythropoietic gene expression was elevated in lineage-depleted spleen cells from Cd47 -/- mice. Expression of several genes associated with early stages of erythropoiesis was elevated in mice lacking CD47 or its signaling ligand thrombospondin-1, consistent with previous evidence that this signaling pathway inhibits expression of multipotent stem cell transcription factors in spleen. In contrast, cells expressing markers of committed erythroid progenitors were more abundant in Cd47 -/- spleens but significantly depleted in Thbs1 -/- spleens. Single-cell transcriptome and flow cytometry analyses indicated that loss of CD47 is associated with accumulation and increased proliferation in spleen of Ter119 - CD34 + progenitors and Ter119 + CD34 - committed erythroid progenitors with elevated mRNA expression of Kit, Ermap, and Tfrc. Induction of committed erythroid precursors is consistent with the known function of CD47 to limit the phagocytic removal of aged erythrocytes. Conversely, loss of thrombospondin-1 delays the turnover of aged red blood cells, which may account for the suppression of committed erythroid precursors in Thbs1 -/- spleens relative to basal levels in wild-type mice. In addition to defining a role for CD47 to limit extramedullary erythropoiesis, these studies reveal a thrombospondin-1-dependent basal level of extramedullary erythropoiesis in adult mouse spleen.
Keyphrases
- gene expression
- single cell
- stem cells
- signaling pathway
- wild type
- transcription factor
- flow cytometry
- magnetic resonance imaging
- dna methylation
- poor prognosis
- metabolic syndrome
- type diabetes
- induced apoptosis
- high fat diet induced
- computed tomography
- rna seq
- insulin resistance
- cell death
- long non coding rna
- bone mineral density