Multicenter prospective evaluation of diagnostic potential of flow cytometric aberrancies in myelodysplastic syndromes by the ELN iMDS flow working group.
Wolfgang KernTheresia M WestersFrauke BellosMarie Christine BenePeter BettelheimLisa Eidenschink BrodersenKate BurburySung-Chao ChuMatthew CullenMatteo Della PortaAlan Stewart DunlopUlrika JohanssonSergio MatarrazUta OelschlaegelKiyoyuki OgataAnna PorwitFrank PreijersKatherina PsarraLeonie SaftDolores SubiráElisabeth WeißVincent H J van der VeldenArjan van de LoosdrechtPublished in: Cytometry. Part B, Clinical cytometry (2022)
Data from this prospective multicenter study led to recognition of 17 immunophenotypic markers allowing to identify cases 'in agreement with MDS'. Moreover, data emphasizes the clinical utility of immunophenotyping in MDS diagnostics, given the high concordance between cytomorphology and the flow cytometric readout. Results from the current study challenge the application of the cytomorphologically defined cut-off of 5% blasts for flow cytometry and rather suggest a 3% cut-off for the latter.