RNase H-dependent DNA thresholder modulated by cancer marker concentration.
Moustapha A Y NourValeriia S DrozdEvgenii A LemeshkoLilia TafranAdeliia A SalimovaAlexandra V KulikovaAhmed A EldeebPublished in: Chemical communications (Cambridge, England) (2024)
Threshold antisense oligonucleotide constructs were designed to cleave mRNA within different biomarker concentrations. The mRNA cleavage is activated by 2.6, 7.5 or 39.5 nM of biomarker depending on the construct design. The constructs can be used to differentiate cancer from normal cells by the level of oncogene expression followed by silencing of a targeted gene.
Keyphrases
- papillary thyroid
- squamous cell
- induced apoptosis
- binding protein
- poor prognosis
- cell cycle arrest
- photodynamic therapy
- gene expression
- circulating tumor
- lymph node metastasis
- squamous cell carcinoma
- single molecule
- genome wide
- copy number
- dna methylation
- cell death
- endoplasmic reticulum stress
- dna binding
- circulating tumor cells