A high spatial resolution osteogenic differentiation in human mesenchymal stem cells induced by femtosecond laser.
Shiyue LiuDihan ChenZhenming XieShirui ZhaoWanyi TangHao HeYi-Ping HoHo-Pui HoSiu-Kai KongPublished in: Journal of biophotonics (2022)
A variety of physical and chemical methods have been developed in research laboratories for the induction of stem cell differentiation. However, the use of exogenous chemicals and materials may limit their widespread utility in clinics. To develop a clean and precise induction approach with minimal invasion, we reported here that 1-second stimulation by a tightly focused femtosecond laser (fsL) (140 mW/μm<sup>2</sup> , 200 fs) can modulate the signaling systems in human mesenchymal cells, such as intracellular calcium and reactive oxygen species. Upon stimulation on an automatic platform, hMSCs were found to express osteoblastic markers and form calcium-rich deposits. Moreover, tissue mineralization was observed when the fsL-illuminated hMSCs were ectopically transplanted into nude mice. Collectively, we described a novel and non-contact optical stimulation method for cell differentiation with high spatiotemporal resolution.
Keyphrases
- mesenchymal stem cells
- stem cells
- reactive oxygen species
- endothelial cells
- bone marrow
- primary care
- umbilical cord
- induced apoptosis
- pluripotent stem cells
- physical activity
- mental health
- deep learning
- single molecule
- cell cycle arrest
- machine learning
- type diabetes
- cell proliferation
- insulin resistance
- vascular smooth muscle cells
- cell death
- high fat diet induced
- skeletal muscle
- mass spectrometry
- high speed
- wild type