Accurate Detection of Rare Mutant Alleles by Target Base-Specific Cleavage with the CRISPR/Cas9 System.
Dongin LeeJi Hyun LeeDuhee BangPublished in: ACS synthetic biology (2021)
The detection of low-frequency somatic mutations enables early diagnosis of disease; however, base-substitution errors that arise during genomic library preparation and high-throughput sequencing can lead to false diagnostic information. To discriminate true genomic alterations from technical errors, we developed spCas9-assisted true variant labeling sequencing (CARVE-seq), which detects low-frequency mutant alleles with high accuracy. CARVE-seq utilizes single-base discrimination during spCas9 cleavage reactions to exclude technical errors. Ten single nucleotide variants that recurrently occur in tumors were assayed by CARVE-seq using 20 ng reference samples, and 100% positive predictive value and specificity was observed, which proved the highly accurate performance of CARVE-seq.
Keyphrases
- single cell
- genome wide
- copy number
- rna seq
- crispr cas
- high throughput sequencing
- patient safety
- adverse drug
- dna methylation
- loop mediated isothermal amplification
- high resolution
- genome editing
- real time pcr
- dna binding
- label free
- emergency department
- health information
- transcription factor
- gene expression
- social media
- mass spectrometry
- quantum dots
- electronic health record
- sensitive detection