Development of drug discovery screening system by molecular interaction kinetics-mass spectrometry.
Naoko ObiTetsuya FukudaNoboru NakayamaJohn ErvinYasuhiko BandoToshihide NishimuraSatoru NagatoishiKouhei TsumotoTakeshi KawamuraPublished in: Rapid communications in mass spectrometry : RCM (2018)
Six small-molecule binders of CAII were analyzed quantitatively using nPOI and MIK-MS, and the results were compared to published surface plasmon resonance (SPR) results. The nPOI and SPR results show good agreement, confirming the reliability of the analysis. Time-dependent binding results may be obtained by our MS sensorgram approach. Drugs that meet medical needs in a short period are required; this nPOI-LC-MS system is considered an important tool for rapid drug discovery.
Keyphrases
- drug discovery
- mass spectrometry
- small molecule
- liquid chromatography
- multiple sclerosis
- ms ms
- gas chromatography
- high performance liquid chromatography
- capillary electrophoresis
- high resolution
- healthcare
- protein protein
- randomized controlled trial
- dna binding
- single molecule
- loop mediated isothermal amplification
- quantum dots
- drug induced
- data analysis