Rapid and sensitive point-of-care detection of Leptospira by RPA-CRISPR/Cas12a targeting lipL32.

Sirawit JirawannapornUmaporn LimothaiSasipha TachaboonJanejira DinhuzenPatcharakorn KiatamornrakWatchadaporn ChaisuriyongJom Bhumitrakul Oraphan Mayuramart Sunchai Payungporn Nattachai Srisawat

Published in: PLoS neglected tropical diseases (2022)

The RPA-CRISPR/Cas12 targeting the lipL32 gene demonstrated acceptable sensitivity and excellent specificity for detection of leptospires. This assay might be an appropriate test for acute leptospirosis screening in limited-resource settings.

Keyphrases

crispr cas
loop mediated isothermal amplification
genome editing
cancer therapy
label free
real time pcr
liver failure
high throughput
sensitive detection
respiratory failure
copy number
genome wide
single cell
genome wide identification
acute respiratory distress syndrome
mechanical ventilation
structural basis