Detection of dengue using PAMAM dendrimer integrated tapered optical fiber sensor.
Yasmin Mustapha KamilSura H Al-RekabiMohd Hanif YaacobAmir SyahirHui Yee CheeMohd Adzir MahdiMuhammad Hafiz Abu BakarPublished in: Scientific reports (2019)
The exponential escalation of dengue cases has indeed become a global health crisis. This work elaborates on the development of a biofunctionalized tapered optical fiber (TOF) based sensor with the integration of polyamidoamine (PAMAM) dendrimer for the detection of dengue E protein. The dimension of the TOF generated an evanescent field that was sensitive to any changes in the external medium while the integration of PAMAM promoted more adhesion of bio-recognition molecules; anti-DENV II E protein antibodies; that were complementary to the targeted protein. This in return created more active sites for the absorption of DENV II E proteins onto the tapered region. The resolution and detection limit of the sensor are 19.53 nm/nM and 1 pM, respectively with Kd = 1.02 × 10-10 M.
Keyphrases
- dengue virus
- zika virus
- global health
- aedes aegypti
- loop mediated isothermal amplification
- public health
- mass spectrometry
- protein protein
- label free
- ms ms
- real time pcr
- high resolution
- photodynamic therapy
- air pollution
- small molecule
- cancer therapy
- drug delivery
- escherichia coli
- quantum dots
- pseudomonas aeruginosa
- polycyclic aromatic hydrocarbons
- water soluble