Experimental tumor growth of canine osteosarcoma cell line on chick embryo chorioallantoic membrane (in vivo studies).
Magdalena WalewskaIzabella DolkaAnna MałekAnna WojtalewiczAgata WojtkowskaArtur ŻbikowskiRoman LechowskiKatarzyna A Zabielska-KoczywąsPublished in: Acta veterinaria Scandinavica (2017)
The chick embryo chorioallantoic membrane (CAM) model is extensively used in human medicine in preclinical oncological studies. The CAM model has several advantages: low cost, simple experimental approach, time saving and following "3R principles". Research has shown that the human osteosarcoma cell lines U2OS, MMNG-HOS, and SAOS can form tumors on the CAM. In veterinary medicine, this has been described only for feline fibrosarcomas, feline mammary carcinomas and canine osteosarcomas. However, in case of canine osteosarcomas, it has been shown that only non-adherent osteosarcoma stem cells isolated from KTOSA5 and CSKOS cell lines have the ability to form microtumors on the CAM after an incubation period of 5 days, in contrast to adherent KTOSA5 and CSKOS cells. In the presented study, we have proven that the commercial adherent canine osteosarcoma cell line (D-17) can form vascularized tumors on the CAM after the incubation period of 10 days.
Keyphrases
- stem cells
- low cost
- endothelial cells
- induced pluripotent stem cells
- induced apoptosis
- magnetic resonance
- pluripotent stem cells
- multidrug resistant
- prostate cancer
- cell therapy
- cell cycle arrest
- rectal cancer
- cell death
- magnetic resonance imaging
- pregnancy outcomes
- pregnant women
- mesenchymal stem cells
- bone marrow
- radical prostatectomy
- computed tomography
- contrast enhanced