Morpho-Functional Effect of a New Collagen-Based Medical Device on Human Gingival Fibroblasts: An In Vitro Study.
Tea RomascoPier Michele MandrilloErica MorsutMargherita TumedeiDomitilla MandatoriMorena PetriniMaria Cristina CuriaFrancesco De AngelisCamillo D'ArcangeloAdriano PiattelliNatalia Di PietroPublished in: Biomedicines (2023)
Maintaining periodontal and peri-implant soft tissues health is crucial for the long-term health of teeth and dental implants. New biomedical strategies aimed at avoiding connective tissue alterations and related diseases (e.g., periodontitis and peri-implantitis) are constantly evolving. Among these, collagen-based medical products have proven to be safe and effective. Accordingly, the aim of the present study was to evaluate the effects of Dental SKIN BioRegulation (Guna S.p.a., Milan, Italy), a new injectable medical device composed of type I collagen of porcine origin, on primary cultures of human gingival fibroblasts (hGF). To this end, hGF were cultured on collagen-coated (COL, 100 µg/2 mL) or uncoated plates (CTRL) before evaluating cell viability (24 h, 48 h, 72 h, and 7 d), wound healing properties (3 h, 6 h, 12 h, 24 h, and 48 h), and the activation of mechanotransduction markers, such as FAK, YAP, and TAZ (48 h). The results proved a significant increase in cell viability at 48 h ( p < 0.05) and wound closure at 24 h ( p < 0.001) of hGF grown on COL, with an increasing trend at all time-points. Furthermore, COL significantly induced the expression of FAK and YAP/TAZ ( p < 0.05), thereby promoting the activation of mechanotransduction signaling pathways. Overall, these data suggest that COL, acting as a mechanical bio-scaffold, could represent a useful treatment for gingival rejuvenation and may possibly help in the resolution of oral pathologies.
Keyphrases
- wound healing
- healthcare
- endothelial cells
- tissue engineering
- high glucose
- public health
- poor prognosis
- induced pluripotent stem cells
- mental health
- health information
- signaling pathway
- electronic health record
- oxidative stress
- long non coding rna
- human health
- health promotion
- cell proliferation
- climate change
- social media
- hyaluronic acid
- machine learning
- data analysis
- soft tissue
- endoplasmic reticulum stress
- platelet rich plasma