Plasma microRNA Profiling in Type 2 Diabetes Mellitus: A Pilot Study.
Ziravard N TonyanYury A BarbitoffYulia A NasykhovaMaria M DanilovaPolina Yu KozyulinaAnastasiia A MikhailovaOlga L BulgakovaMargarita E VlasovaNikita V GolovkinAndrey S GlotovPublished in: International journal of molecular sciences (2023)
Type 2 diabetes mellitus (T2D) is a chronic metabolic disease characterized by insulin resistance and β-cell dysfunction and leading to many micro- and macrovascular complications. In this study we analyzed the circulating miRNA expression profiles in plasma samples from 44 patients with T2D and 22 healthy individuals using next generation sequencing and detected 229 differentially expressed miRNAs. An increased level of miR-5588-5p, miR-125b-2-3p, miR-1284, and a reduced level of miR-496 in T2D patients was verified. We also compared the expression landscapes in the same group of patients depending on body mass index and identified differential expression of miR-144-3p and miR-99a-5p in obese individuals. Identification and functional analysis of putative target genes was performed for miR-5588-5p, miR-125b-2-3p, miR-1284, and miR-496, showing chromatin modifying enzymes and apoptotic genes being among the significantly enriched pathways.
Keyphrases
- cell proliferation
- long non coding rna
- end stage renal disease
- long noncoding rna
- chronic kidney disease
- insulin resistance
- newly diagnosed
- ejection fraction
- type diabetes
- genome wide
- adipose tissue
- peritoneal dialysis
- poor prognosis
- metabolic syndrome
- prognostic factors
- single cell
- gene expression
- cell death
- dna methylation
- transcription factor
- oxidative stress
- stem cells
- risk factors
- bariatric surgery
- polycystic ovary syndrome
- bioinformatics analysis
- weight loss
- genome wide identification