Endo- and Exometabolome Crosstalk in Mesenchymal Stem Cells Undergoing Osteogenic Differentiation.
Daniela S C BispoLenka MichálkováMarlene CorreiaCatarina S H JesusIola F DuarteBrian J GoodfellowMariana B OliveiraJoao F ManoAna I GilPublished in: Cells (2022)
This paper describes, for the first time to our knowledge, a lipidome and exometabolome characterization of osteogenic differentiation for human adipose tissue stem cells (hAMSCs) using nuclear magnetic resonance (NMR) spectroscopy. The holistic nature of NMR enabled the time-course evolution of cholesterol, mono- and polyunsaturated fatty acids (including ω-6 and ω-3 fatty acids), several phospholipids (phosphatidylcholine, phosphatidylethanolamine, sphingomyelins, and plasmalogens), and mono- and triglycerides to be followed. Lipid changes occurred almost exclusively between days 1 and 7, followed by a tendency for lipidome stabilization after day 7. On average, phospholipids and longer and more unsaturated fatty acids increased up to day 7, probably related to plasma membrane fluidity. Articulation of lipidome changes with previously reported polar endometabolome profiling and with exometabolome changes reported here in the same cells, enabled important correlations to be established during hAMSC osteogenic differentiation. Our results supported hypotheses related to the dynamics of membrane remodelling, anti-oxidative mechanisms, protein synthesis, and energy metabolism. Importantly, the observation of specific up-taken or excreted metabolites paves the way for the identification of potential osteoinductive metabolites useful for optimized osteogenic protocols.
Keyphrases
- mesenchymal stem cells
- fatty acid
- magnetic resonance
- umbilical cord
- bone marrow
- stem cells
- adipose tissue
- cell therapy
- ms ms
- induced apoptosis
- endothelial cells
- healthcare
- insulin resistance
- high resolution
- metabolic syndrome
- cell cycle arrest
- oxidative stress
- high fat diet
- mass spectrometry
- magnetic resonance imaging
- endoplasmic reticulum stress
- climate change