Surfactant Lipidomics of Alveolar Lavage Fluid in Mice Based on Ultra-High-Performance Liquid Chromatography Coupled to Hybrid Quadrupole-Exactive Orbitrap Mass Spectrometry.
Rui YangYing ZhangWenjuan QianLinxiu PengLili LinJia XuTong XieJianjian JiXiuqin ZhanJin Jun ShanPublished in: Metabolites (2019)
Surfactant lipid metabolism is closely related to pulmonary diseases. Lipid metabolism disorder can cause lung diseases, vice versa. With this rationale, a useful method was established in this study to determine the lipidome in bronchoalveolar lavage fluid (BALF) of mice. The lipid components in BALF were extracted by liquid-liquid extraction (methanol and methyl tert-butyl ether, and water). Ultra-high-performance liquid chromatography coupled to hybrid Quadrupole-Exactive Orbitrap mass spectrometry was used to analyze the extracted samples, which showed a broad scanning range of 215-1800 m/z. With MS-DIAL software and built-in LipidBlast database, we identified 38 lipids in positive, and 31 lipids in negative, ion mode, including lysophosphatidylcholine (lysoPC), phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylglycerol (PG), etc. Then, the changes of lipids in BALF of mice with acute lung injury (ALI) induced by lipopolysaccharide (LPS) was investigated, which may contribute to further exploration of the pathogenesis of ALI.
Keyphrases
- ultra high performance liquid chromatography
- tandem mass spectrometry
- liquid chromatography
- mass spectrometry
- high resolution mass spectrometry
- gas chromatography
- high performance liquid chromatography
- simultaneous determination
- high resolution
- fatty acid
- solid phase extraction
- high fat diet induced
- capillary electrophoresis
- inflammatory response
- ms ms
- lps induced
- emergency department
- multiple sclerosis
- pulmonary hypertension
- anti inflammatory
- toll like receptor
- clinical trial
- insulin resistance
- metabolic syndrome
- ionic liquid