Targeting USP-7 by a Novel Fluorinated 5-Pyrazolyl-Urea Derivative.
Elva MorrettaChiara BrulloRaffaella BelvedereAntonello PetrellaAndrea SpallarossaMaria Chiara MontiPublished in: International journal of molecular sciences (2023)
The impact of innovative technologies on the target discovery has been employed here to characterize the interactome of STIRUR 41, a promising 3-fluoro-phenyl-5-pyrazolyl-urea derivative endowed with anti-cancer activity, on neuroblastoma-related cells. A drug affinity responsive target stability-based proteomic platform has been optimized to elucidate the molecular mechanism at the basis of STIRUR 41 action, together with immunoblotting analysis and in silico molecular docking. Ubiquitin Specific Protease 7 (USP-7), one of the deubiquitinating enzymes which protect substrate proteins from proteasomal degradation, has been identified as the most affine STIRUR 41 target. As further demonstrated by in vitro and in-cell assays, STIRUR 41 was able to inhibit both the enzymatic activity of USP-7 and its expression levels in neuroblastoma-related cells, thus laying an encouraging base for the blockade of USP-7 downstream signaling.
Keyphrases
- molecular docking
- induced apoptosis
- cell cycle arrest
- high throughput
- molecular dynamics simulations
- small molecule
- poor prognosis
- emergency department
- endoplasmic reticulum stress
- cancer therapy
- computed tomography
- drug delivery
- heat stress
- oxidative stress
- positron emission tomography
- cell death
- electronic health record
- pet ct