Dual-Mode Detection of Bacterial 16S Ribosomal RNA in Tissues.
Shengnan XuQingyang ZhangXiaowan HanYali WangXiang WangMarc NazareJian-Dong JiangHai-Yu HuPublished in: ACS sensors (2020)
The specific detection of pathogens has long been recognized as a vital strategy for controlling bacterial infections. Herein, a novel hydrophilic aromatic-imide-based thermally activated delayed fluorescence (TADF) probe, AI-Cz-Neo, is designed and synthesized by the conjugation of a TADF emitter with a bacterial 16S ribosomal RNA-targeted moiety, neomycin. Biological data showed for the first time that AI-Cz-Neo could be successfully applied for the dual-mode detection of bacterial 16S rRNA using confocal fluorescence imaging and time-resolved fluorescence imaging (TRFI) in both cells and tissues. These findings greatly expand the application of TADF fluorophores in time-resolved biological imaging and provide a promising strategy for the precise and reliable diagnosis of bacterial infections based on the dual-mode imaging of bacterial 16S rRNA by fluorescence intensity and fluorescence lifetime.
Keyphrases
- fluorescence imaging
- photodynamic therapy
- high resolution
- single molecule
- gene expression
- loop mediated isothermal amplification
- induced apoptosis
- high intensity
- energy transfer
- drug delivery
- mass spectrometry
- oxidative stress
- signaling pathway
- deep learning
- liquid chromatography
- cancer therapy
- electronic health record
- raman spectroscopy