Role of endothelial colony forming cells (ECFCs) Tetrahydrobiopterin (BH4) in determining ECFCs functionality in coronary artery disease (CAD) patients.
Atanu SenArchna SinghAmbuj RoySujata MohantyNitish NaikMani KalaivaniLakshmy RamakrishnanPublished in: Scientific reports (2022)
Nitric oxide (NO . ) is critical for functionality of endothelial colony forming cells (ECFCs). Dimerization of endothelial nitric oxide synthase (eNOS) is must to produce NO . and tetrahydrobiopterin (BH4) plays a crucial role in stabilizing this state. We investigated BH4 level in ECFCs and its effect on ECFCs functionality in CAD patients. Intracellular biopterin levels and ECFCs functionality in terms of cell viability, adhesion, proliferation, in vitro wound healing and angiogenesis were assessed. Guanosine Triphosphate Cyclohydrolase-1 (GTPCH-1) expression was studied in ECFCs. Serum total reactive oxygen/nitrogen species was measured and effect of nitrosative stress on ECFC's biopterins level and functionality were evaluated by treating with 3-morpholino sydnonimine (SIN-1). BH4 level was significantly lower in ECFCs from CAD patients. Cell proliferation, wound closure reflecting cellular migration as well as in vitro angiogenesis were impaired in ECFCs from CAD patients. Wound healing capacity and angiogenesis were positively correlated with ECFC's BH4. A negative effect of nitrosative stress on biopterins level and cell functionality was observed in SIN-1 treated ECFCs. ECFCs from CAD exhibited impaired functionality and lower BH4 level. Association of BH4 with wound healing capacity and angiogenesis suggest its role in maintaining ECFC's functionality. Oxidative stress may be a determinant of intracellular biopterin levels.
Keyphrases
- coronary artery disease
- end stage renal disease
- wound healing
- nitric oxide
- endothelial cells
- ejection fraction
- chronic kidney disease
- cell proliferation
- nitric oxide synthase
- prognostic factors
- induced apoptosis
- stem cells
- high resolution
- single cell
- staphylococcus aureus
- poor prognosis
- type diabetes
- dna damage
- mass spectrometry
- patient reported outcomes
- signaling pathway
- pseudomonas aeruginosa
- atrial fibrillation
- biofilm formation
- acute coronary syndrome
- escherichia coli
- left ventricular
- cystic fibrosis
- candida albicans
- heat shock
- binding protein
- coronary artery bypass grafting