MicroRNA-19a attenuates hypoxia-induced cardiomyocyte apoptosis by downregulating NHE-1 expression and decreasing calcium overload.
Jiaqi MaZhangwei ChenYuanji MaYan XiaKai HuYou ZhouAo ChenJun-Bo GeJunbo GePublished in: Journal of cellular biochemistry (2019)
miR-19a has been shown to be involved in coronary microvascular obstruction injury; however, the underlying molecular mechanisms remain unknown. In our study, we tried to explore the role of miR-19a in cardiomyocyte apoptosis and calcium overload in vivo and in vitro induced by hypoxia. We established the acute myocardial infarction (AMI) rat model by ligating the left anterior descending artery. The expression of miR-19a in the infarct zone of AMI rats and myocardial tissue in the same position in sham rats was analyzed using RT-qPCR while Na(+) /H(+) exchanger 1 (NHE-1) was detected by Western blotting. We also observed the effects of overexpressing miR-19a or administering an NHE-1 inhibitor (cariporide) on hypoxia-induced (HI) calcium overload and apoptosis in primary cardiomyocytes. In addition, dual-luciferase reporter assays were conducted to investigate the potential target of miR-19a on NHE-1. Decreased miR-19a expression, as well as increased apoptosis and NHE-1 expression, were observed in the AMI model. Furthermore, after hypoxia stimulation, miR-19a was gradually reduced as time increased in primary cardiomyocytes. Overexpressing miR-19a using mimics ameliorated the increase in NHE-1 in hypoxic cardiomyocytes and thereby reduced the HI cell calcium overload and cell apoptosis rate from 12.32% to 9.5% (P < .01). In addition, the dual-luciferase reporter gene assay results verified that NHE-1 was the direct target of miR-19a. Our findings suggest that miR-19a activation can attenuate HI cardiomyocyte apoptosis by downregulating NHE-1 expression and decreasing calcium overload.
Keyphrases
- cell proliferation
- long non coding rna
- poor prognosis
- long noncoding rna
- acute myocardial infarction
- oxidative stress
- endoplasmic reticulum stress
- cell cycle arrest
- cell death
- left ventricular
- binding protein
- coronary artery disease
- coronary artery
- crispr cas
- high throughput
- clinical trial
- heart failure
- south africa
- endothelial cells
- atrial fibrillation
- dna methylation
- climate change
- copy number
- mesenchymal stem cells
- transcatheter aortic valve replacement
- double blind
- human health
- cell therapy