Genome-Wide Identification and Expression Analysis of SlNRAMP Genes in Tomato under Nutrient Deficiency and Cadmium Stress during Arbuscular Mycorrhizal Symbiosis.
Junli LiuXiaoqi BaoGaoyang QiuHua LiYuan WangXiaodong ChenQinglin FuBin GuoPublished in: International journal of molecular sciences (2024)
Arbuscular mycorrhizal (AM) fungi are well known for enhancing phosphorus uptake in plants; however, their regulating roles in cation transporting gene family, such as natural resistance-associated macrophage protein (NRAMP), are still limited. Here, we performed bioinformatics analysis and quantitative expression assays of tomato SlNRAMP 1 to 5 genes under nutrient deficiency and cadmium (Cd) stress in response to AM symbiosis. These five SlNRAMP members are mainly located in the plasma or vacuolar membrane and can be divided into two subfamilies. Cis-element analysis revealed several motifs involved in phytohormonal and abiotic regulation in their promoters. SlNRAMP2 was downregulated by iron deficiency, while SlNRAMP1 , SlNRAMP3 , SlNRAMP4, and SlNRAMP5 responded positively to copper-, zinc-, and manganese-deficient conditions. AM colonization reduced Cd accumulation and expression of SlNRAMP3 but enhanced SlNRAMP1 , SlNRAMP2, and SlNRMAP4 in plants under Cd stress. These findings provide valuable genetic information for improving tomato resilience to nutrient deficiency and heavy metal stress by developing AM symbiosis.
Keyphrases
- genome wide identification
- bioinformatics analysis
- heavy metals
- transcription factor
- poor prognosis
- genome wide
- stress induced
- iron deficiency
- oxide nanoparticles
- risk assessment
- binding protein
- depressive symptoms
- sewage sludge
- health risk
- single cell
- dna methylation
- long non coding rna
- heat stress
- health risk assessment
- copy number